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Author (up) Ambrosone, A.; Costa, A.; Leone, A.; Grillo, S. file  url
doi  openurl
  Title Beyond transcription: RNA-binding proteins as emerging regulators of plant response to environmental constraints Type Journal Article
  Year 2012 Publication Plant Science : an International Journal of Experimental Plant Biology Abbreviated Journal Plant Sci  
  Volume 182 Issue Pages 12-18  
  Keywords Abscisic Acid/metabolism; Acclimatization/*physiology; Gene Expression Regulation, Plant; Osmotic Pressure/physiology; *Plant Physiological Processes; Plants/genetics; RNA-Binding Proteins/genetics/metabolism/*physiology; Transcription, Genetic  
  Abstract RNA-binding proteins (RBPs) govern many aspects of RNA metabolism, including pre-mRNA processing, transport, stability/decay and translation. Although relatively few plant RNA-binding proteins have been characterized genetically and biochemically, more than 200 RBP genes have been predicted in Arabidopsis and rice genomes, suggesting that they might serve specific plant functions. Besides their role in normal cellular functions, RBPs are emerging also as an interesting class of proteins involved in a wide range of post-transcriptional regulatory events that are important in providing plants with the ability to respond rapidly to changes in environmental conditions. Here, we review the most recent results and evidence on the functional role of RBPs in plant adaptation to various unfavourable environmental conditions and their contribution to enhance plant tolerance to abiotic stresses, with special emphasis on osmotic and temperature stress.  
  Call Number Serial 1226  
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Author (up) Burgos, A.; Szymanski, J.; Seiwert, B.; Degenkolbe, T.; Hannah, M.A.; Giavalisco, P.; Willmitzer, L. file  url
  Title Analysis of short-term changes in the Arabidopsis thaliana glycerolipidome in response to temperature and light Type Journal Article
  Year 2011 Publication The Plant Journal: for Cell and Molecular Biology Abbreviated Journal Plant J  
  Volume 66 Issue 4 Pages 656-668  
  Keywords Arabidopsis/*metabolism; Biosynthetic Pathways; Fatty Acids/metabolism; Galactolipids/analysis/metabolism; *Light; *Lipid Metabolism; Phosphatidylcholines/analysis/metabolism; Phosphatidylethanolamines/analysis/metabolism; Phosphatidylinositols/analysis/metabolism; Plant Leaves/*metabolism; *Temperature  
  Abstract Although the influence of temperature, particularly cold, on lipid metabolism is well established, previous studies have focused on long-term responses and have largely ignored the influence of other interacting environmental factors. Here, we present a time-resolved analysis of the early responses of the glycerolipidome of Arabidopsis thaliana plants exposed to various temperatures (4, 21 and 32 degrees C) and light intensities (darkness, 75, 150 and 400 mumol m(-2) s(-1)), including selected combinations. Using a UPLC/MS-based lipidomic platform, we reproducibly measured most glycerolipid species reported for Arabidopsis leaves, including the classes phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI) phosphatidylglycerol (PG), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and sulfoquinovosyldiacylglycerol (SQDG). In addition to known lipids, we have identified previously unobserved compounds, such as 36-C PGs and eukaryotic phospholipids containing 16:3 acyl chains. Occurrence of these lipid species implies the action of new biochemical mechanisms. Exposition of Arabidopsis plants to various light and temperature regimes results in two major effects. The first is the dependence of the saturation level of PC and MGDG pools on light intensity, likely arising from light regulation of de novo fatty acid synthesis. The second concerns an immediate decrease in unsaturated species of PG at high-temperature conditions (32 degrees C), which could mark the first stages of adaptation to heat-stress conditions. Observed changes are discussed in the context of current knowledge, and new hypotheses have been formulated concerning the early stages of the plant response to changing light and temperature conditions.  
  Call Number Serial 302  
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Author (up) Ghars, M.A.; Parre, E.; Debez, A.; Bordenave, M.; Richard, L.; Leport, L.; Bouchereau, A.; Savoure, A.; Abdelly, C. url  openurl
  Title Comparative salt tolerance analysis between Arabidopsis thaliana and Thellungiella halophila, with special emphasis on K(+)/Na(+) selectivity and proline accumulation Type Journal Article
  Year 2008 Publication Journal of Plant Physiology Abbreviated Journal J Plant Physiol  
  Volume 165 Issue 6 Pages 588-599  
  Keywords Arabidopsis/drug effects/growth & development/*metabolism; Biomass; Brassicaceae/drug effects/growth & development/*metabolism; Potassium/*metabolism; Proline/*metabolism; Salinity; *Salt-Tolerance/drug effects; Sodium/*metabolism; Sodium Chloride/pharmacology; Stress, Physiological/drug effects; Water/metabolism  
  Abstract The eco-physiology of salt tolerance, with an emphasis on K(+) nutrition and proline accumulation, was investigated in the halophyte Thellungiella halophila and in both wild type and eskimo-1 mutant of the glycophyte Arabidopsis thaliana, which differ in their proline accumulation capacity. Plants cultivated in inert sand were challenged for 3 weeks with up to 500mM NaCl. Low salinity significantly decreased A. thaliana growth, whereas growth restriction was significant only at salt concentrations equal to or exceeding 300mM NaCl in T. halophila. Na(+) content generally increased with the amount of salt added in the culture medium in both species, but T. halophila showed an ability to control Na(+) accumulation in shoots. The analysis of the relationship between water and Na(+) contents suggested an apoplastic sodium accumulation in both species; this trait was more pronounced in A. thaliana than in T. halophila. The better NaCl tolerance in the latter was associated with a better K(+) supply, resulting in higher K(+)/Na(+) ratios. It was also noteworthy that, despite highly accumulating proline, the A. thaliana eskimo-1 mutant was the most salt-sensitive species. Taken together, our findings indicate that salt tolerance may be partly linked to the plants' ability to control Na(+) influx and to ensure appropriate K(+) nutrition, but is not linked to proline accumulation.  
  Call Number Serial 230  
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Author (up) Kim, D.S.; Cho, D.S.; Park, W.-M.; Na, H.J.; Nam, H.G. file  url
  Title Proteomic pattern-based analyses of light responses in Arabidopsis thaliana wild-type and photoreceptor mutants Type Journal Article
  Year 2006 Publication Proteomics Abbreviated Journal Proteomics  
  Volume 6 Issue 10 Pages 3040-3049  
  Keywords Arabidopsis/metabolism/*radiation effects; Arabidopsis Proteins/*biosynthesis; Cluster Analysis; Electrophoresis, Gel, Two-Dimensional; *Light; Photosynthetic Reaction Center Complex Proteins/*genetics/physiology; Phytochrome/genetics/physiology; Proteome/*biosynthesis; Signal Transduction  
  Abstract Light critically affects the physiology of plants. Using two-dimensional gel electrophoresis, we used a proteomics approach to analyze the responses of Arabidopsis thaliana to red (660 nm), far-red (730 nm) and blue (450 nm) light, which are utilized by type II and type I phytochromes, and blue light receptors, respectively. Under specific light treatments, the proteomic profiles of 49 protein spots exhibited over 1.8-fold difference in protein abundance, significant at p <0.05. Most of these proteins were metabolic enzymes, indicating metabolic changes induced by light of specific wavelengths. The differentially-expressed proteins formed seven clusters, reflecting co-regulation. We used the 49 differentially-regulated proteins as molecular markers for plant responses to light, and by developing a procedure that calculates the Pearson correlation distance of cluster-to-cluster similarity in expression changes, we assessed the proteome-based relatedness of light responses for wild-type and phytochrome mutant plants. Overall, this assessment was consistent with the known physiological responses of plants to light. However, we also observed a number of novel responses at the proteomic level, which were not predicted from known physiological changes.  
  Call Number Serial 275  
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Author (up) Kosova, K.; Vitamvas, P.; Prasil, I.T.; Renaut, J. file  url
  Title Plant proteome changes under abiotic stress--contribution of proteomics studies to understanding plant stress response Type Journal Article
  Year 2011 Publication Journal of Proteomics Abbreviated Journal J Proteomics  
  Volume 74 Issue 8 Pages 1301-1322  
  Keywords Arabidopsis/genetics; Cold Temperature/adverse effects; Droughts; Gene Expression Profiling; Herbicides/pharmacology; Hot Temperature/adverse effects; Metals, Heavy/adverse effects; Oryza sativa/genetics; Plant Proteins/*genetics; Plants/*genetics; Protein Processing, Post-Translational; Proteome/*genetics; Stress, Physiological/*genetics  
  Abstract Plant acclimation to stress is associated with profound changes in proteome composition. Since proteins are directly involved in plant stress response, proteomics studies can significantly contribute to unravel the possible relationships between protein abundance and plant stress acclimation. In this review, proteomics studies dealing with plant response to a broad range of abiotic stress factors--cold, heat, drought, waterlogging, salinity, ozone treatment, hypoxia and anoxia, herbicide treatments, inadequate or excessive light conditions, disbalances in mineral nutrition, enhanced concentrations of heavy metals, radioactivity and mechanical wounding are discussed. Most studies have been carried out on model plants Arabidopsis thaliana and rice due to large protein sequence databases available; however, the variety of plant species used for proteomics analyses is rapidly increasing. Protein response pathways shared by different plant species under various stress conditions (glycolytic pathway, enzymes of ascorbate-glutathione cycle, accumulation of LEA proteins) as well as pathways unique to a given stress are discussed. Results from proteomics studies are interpreted with respect to physiological factors determining plant stress response. In conclusion, examples of application of proteomics studies in search for protein markers underlying phenotypic variation in physiological parameters associated with plant stress tolerance are given.  
  Call Number Serial 229  
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