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Author (up) Burgos, A.; Szymanski, J.; Seiwert, B.; Degenkolbe, T.; Hannah, M.A.; Giavalisco, P.; Willmitzer, L. file  url
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  Title Analysis of short-term changes in the Arabidopsis thaliana glycerolipidome in response to temperature and light Type Journal Article
  Year 2011 Publication The Plant Journal: for Cell and Molecular Biology Abbreviated Journal Plant J  
  Volume 66 Issue 4 Pages 656-668  
  Keywords Arabidopsis/*metabolism; Biosynthetic Pathways; Fatty Acids/metabolism; Galactolipids/analysis/metabolism; *Light; *Lipid Metabolism; Phosphatidylcholines/analysis/metabolism; Phosphatidylethanolamines/analysis/metabolism; Phosphatidylinositols/analysis/metabolism; Plant Leaves/*metabolism; *Temperature  
  Abstract Although the influence of temperature, particularly cold, on lipid metabolism is well established, previous studies have focused on long-term responses and have largely ignored the influence of other interacting environmental factors. Here, we present a time-resolved analysis of the early responses of the glycerolipidome of Arabidopsis thaliana plants exposed to various temperatures (4, 21 and 32 degrees C) and light intensities (darkness, 75, 150 and 400 mumol m(-2) s(-1)), including selected combinations. Using a UPLC/MS-based lipidomic platform, we reproducibly measured most glycerolipid species reported for Arabidopsis leaves, including the classes phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI) phosphatidylglycerol (PG), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and sulfoquinovosyldiacylglycerol (SQDG). In addition to known lipids, we have identified previously unobserved compounds, such as 36-C PGs and eukaryotic phospholipids containing 16:3 acyl chains. Occurrence of these lipid species implies the action of new biochemical mechanisms. Exposition of Arabidopsis plants to various light and temperature regimes results in two major effects. The first is the dependence of the saturation level of PC and MGDG pools on light intensity, likely arising from light regulation of de novo fatty acid synthesis. The second concerns an immediate decrease in unsaturated species of PG at high-temperature conditions (32 degrees C), which could mark the first stages of adaptation to heat-stress conditions. Observed changes are discussed in the context of current knowledge, and new hypotheses have been formulated concerning the early stages of the plant response to changing light and temperature conditions.  
  Call Number Serial 302  
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Author (up) Diaz de Toranzo, E.G.; Castro, J.A.; Franke de Cazzulo, B.M.; Cazzulo, J.J. file  url
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  Title Interaction of benznidazole reactive metabolites with nuclear and kinetoplastic DNA, proteins and lipids from Trypanosoma cruzi Type Journal Article
  Year 1988 Publication Experientia Abbreviated Journal Experientia  
  Volume 44 Issue 10 Pages 880-881  
  Keywords Animals; Cell Nucleus/analysis; DNA/*metabolism; *Lipid Metabolism; Nitroimidazoles/*metabolism; Proteins/*metabolism; Trypanosoma cruzi/*analysis  
  Abstract Epimastigotes of Trypanosoma cruzi (Tulahuen strain Tul 0 stock) biotransform benznidazole (N-benzyl-2-nitro-1-imidazole acetamide) to reactive metabolites that bind covalently to DNA, proteins and lipids of the parasite. These effects might be related to the trypanocidal action of benznidazole, a chemotherapeutic agent against Chagas' disease.  
  Call Number Serial 556  
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