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Author (up) Foltz, D.R.; Jansen, L.E.T.; Black, B.E.; Bailey, A.O.; Yates, J.R. 3rd; Cleveland, D.W. url  doi
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  Title The human CENP-A centromeric nucleosome-associated complex Type Journal Article
  Year 2006 Publication Nature Cell Biology Abbreviated Journal Nat Cell Biol  
  Volume 8 Issue 5 Pages 458-469  
  Keywords Amino Acid Sequence; Autoantigens--chemistry, isolation & purification; metabolism; Centromere--metabolism; Chromatin Assembly Factor-1; Chromatin Assembly and Disassembly--genetics; Chromosomal Proteins, Non-Histone--chemistry, isolation & purification, metabolism; Chromosomes, Human genetics; DNA-Binding Proteins--metabolism; HeLa Cells; Histones--chemistry; Humans; Mitosis--genetics; Molecular Sequence Data; Nucleosomes--metabolism; Protein Binding; Signal Transduction  
  Abstract The basic element for chromosome inheritance, the centromere, is epigenetically determined in mammals. The prime candidate for specifying centromere identity is the array of nucleosomes assembled with CENP-A, the centromere-specific histone H3 variant. Here, we show that CENP-A nucleosomes directly recruit a proximal CENP-A nucleosome associated complex (NAC) comprised of three new human centromere proteins (CENP-M, CENP-N and CENP-T), along with CENP-U(50), CENP-C and CENP-H. Assembly of the CENP-A NAC at centromeres is dependent on CENP-M, CENP-N and CENP-T. Facilitates chromatin transcription (FACT) and nucleophosmin-1 (previously implicated in transcriptional chromatin remodelling and as a multifunctional nuclear chaperone, respectively) are absent from histone H3-containing nucleosomes, but are stably recruited to CENP-A nucleosomes independent of CENP-A NAC. Seven new CENP-A-nucleosome distal (CAD) centromere components (CENP-K, CENP-L, CENP-O, CENP-P, CENP-Q, CENP-R and CENP-S) are identified as assembling on the CENP-A NAC. The CENP-A NAC is essential, as disruption of the complex causes errors of chromosome alignment and segregation that preclude cell survival despite continued centromere-derived mitotic checkpoint signalling.  
  Call Number Serial 13  
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