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Author Lal, D.; Keim, P.; Delisle, J.; Barker, B.; Rank, M.A.; Chia, N.; Schupp, J.M.; Gillece, J.D.; Cope, E.K. file  url
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Title Mapping and comparing bacterial microbiota in the sinonasal cavity of healthy, allergic rhinitis, and chronic rhinosinusitis subjects Type Journal Article
Year 2017 Publication International Forum of Allergy & Rhinology Abbreviated Journal Int Forum Allergy Rhinol  
Volume 7 Issue 6 Pages 561-569  
Keywords allergic rhinitis; bacteriology; chronic rhinosinusitis; inferior meatus; microbioata; microbiome; middle meatus; nasal polyposis; regional sinonasal microbiota and rhinosinusitis  
Abstract BACKGROUND: The role of microbiota in sinonasal inflammation can be further understood by targeted sampling of healthy and diseased subjects. We compared the microbiota of the middle meatus (MM) and inferior meatus (IM) in healthy, allergic rhinitis (AR), and chronic rhinosinusitis (CRS) subjects to characterize intrasubject, intersubject, and intergroup differences. METHODS: Subjects were recruited in the office, and characterized into healthy, AR, and CRS groups. Endoscopically-guided swab samples were obtained from the MM and IM bilaterally. Bacterial microbiota were characterized by sequencing the V3-V4 region of the 16S ribosomal RNA (rRNA) gene. RESULTS: Intersubject microbiome analyses were conducted in 65 subjects: 8 healthy, 11 AR, and 46 CRS (25 CRS with nasal polyps [CRSwNP]; 21 CRS without nasal polyps [CRSsNP]). Intrasubject analyses were conducted for 48 individuals (4 controls, 11 AR, 8 CRSwNP, and 15 CRSwNP). There was considerable intersubject microbiota variability, but intrasubject profiles were similar (p = 0.001, nonparametric t test). Intrasubject bacterial diversity was significantly reduced in MM of CRSsNP subjects compared to IM samples (p = 0.022, nonparametric t test). CRSsNP MM samples were enriched in Streptococcus, Haemophilus, and Fusobacterium spp. but exhibited loss of diversity compared to healthy, CRSwNP, and AR subject-samples (p < 0.05; nonparametric t test). CRSwNP patients were enriched in Staphylococcus, Alloiococcus, and Corynebacterium spp. CONCLUSION: This study presents the sinonasal microbiome profile in one of the larger populations of non-CRS and CRS subjects, and is the first office-based cohort in the literature. In contrast to healthy, AR, and CRSwNP subjects, CRSsNP MM samples exhibited decreased microbiome diversity and anaerobic enrichment. CRSsNP MM samples had reduced diversity compared to same-subject IM samples, a novel finding.  
Call Number Serial 2174  
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Author Bennett, J.W.; Bentley, R. file  url
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Title Seeing red: the story of prodigiosin Type Journal Article
Year 2000 Publication Advances in Applied Microbiology Abbreviated Journal Adv Appl Microbiol  
Volume 47 Issue Pages 1-32  
Keywords Bacteriology/history; Bread/microbiology; History, 18th Century; History, 19th Century; History, 20th Century; Pigments, Biological/*chemistry/history/metabolism/pharmacology; Prodigiosin/*chemistry/metabolism/pharmacology; Serratia marcescens/*chemistry/metabolism  
Abstract S. marcescens has played an important role in the history of bacterial taxonomy, in research on the transmission of bacterial aerosols, in the study of emerging nosocomial infections, and in the understanding of secondary metabolite biosynthesis. The prodigiosin pigments have intrigued organic chemists and pharmacologists, and play roles in the treatment of infectious diseases such as malaria, and perhaps as immunosuppressant agents. Undecylprodiginine played an important role in the first cloning of a gene, playing a defined role in the biosynthesis of an antibiotic. An O-methyltransferase gene was isolated by complementation and the color of undecylprodiginine was used as the selectable phenotype. The regulation of prodigiosin biosynthesis is complex, being influenced by increased glucose levels and decreased by increased phosphate level. The antibiotic resistance of many strains of S. marcescens is a serious problem with rapid horizontal transfer of drug resistance by plasmids.  
Call Number Serial 1639  
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