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Author (up) Guerra, F.E.; Addison, C.B.; de Jong, N.W.M.; Azzolino, J.; Pallister, K.B.; van Strijp, J.A.G.; Voyich, J.M. file  url
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  Title Staphylococcus aureus SaeR/S-regulated factors reduce human neutrophil reactive oxygen species production Type Journal Article
  Year 2016 Publication Journal of Leukocyte Biology Abbreviated Journal J Leukoc Biol  
  Volume 100 Issue 5 Pages 1005-1010  
  Keywords bacteria; host-pathogen interactions; innate immunity; two-component systems  
  Abstract Neutrophils are the first line of defense after a pathogen has breached the epithelial barriers, and unimpaired neutrophil functions are essential to clear infections. Staphylococcus aureus is a prevalent human pathogen that is able to withstand neutrophil killing, yet the mechanisms used by S. aureus to inhibit neutrophil clearance remain incompletely defined. The production of reactive oxygen species (ROS) is a vital neutrophil antimicrobial mechanism. Herein, we test the hypothesis that S. aureus uses the SaeR/S two-component gene regulatory system to produce virulence factors that reduce neutrophil ROS production. With the use of ROS probes, the temporal and overall production of neutrophil ROS was assessed during exposure to the clinically relevant S. aureus USA300 (strain LAC) and its isogenic mutant LACDeltasaeR/S Our results demonstrated that SaeR/S-regulated factors do not inhibit neutrophil superoxide (O2-) production. However, subsequent neutrophil ROS production was significantly reduced during exposure to LAC compared with LACDeltasaeR/S In addition, neutrophil H2O2 production was reduced significantly by SaeR/S-regulated factors by a mechanism independent of catalase. Consequently, the reduction in neutrophil H2O2 resulted in decreased production of the highly antimicrobial agent hypochlorous acid/hypochlorite anion (HOCl/-OCl). These findings suggest a new evasion strategy used by S. aureus to diminish a vital neutrophil antimicrobial mechanism.  
  Call Number Serial 1704  
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