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Author Moon, J.L.; Banbula, A.; Oleksy, A.; Mayo, J.A.; Travis, J. file  url
  Title Isolation and characterization of a highly specific serine endopeptidase from an oral strain of Staphylococcus epidermidis Type Journal Article
  Year (down) 2001 Publication Biological Chemistry Abbreviated Journal Biol Chem  
  Volume 382 Issue 7 Pages 1095-1099  
  Keywords Amino Acid Sequence; Animals; Cattle; Complement C--chemistry; Electrophoresis, Polyacrylamide Gel; Fibrinogen--chemistry; Humans; Keratins--chemistry; Molecular Sequence Data; Molecular Weight; Mouth Diseases--microbiology; Protease Inhibitors--chemistry; Sequence Homology, Amino Acid; Serine Endopeptidases--chemistry, isolation & purification, metabolism; Staphylococcal Infections--microbiology; Staphylococcus epidermidis--enzymology  
  Abstract Infection by Staphylococcus epidermidis, an opportunistic pathogen, has become a major problem due to the increased use of implanted medical devices and the growing number of patients who are therapeutically or infectiously immunosuppressed. These infections appear to proceed via modulation of the coagulation and complement systems. In this communication we describe the purification and characterization of a novel extracellular proteinase from an oral strain of S. epidermidis that can degrade fibrinogen, complement protein C5, and several other proteins. This proteinase has a strong preference for cleavage after glutamic acid residues, but not after aspartic acid. The S. epidermidis enzyme may be a multifunctional protein which not only provides this organism with both the ability to evade the complement defense system and to dysregulate the coagulation cascade, but also supplies nutrients for its growth through the degradation of Glu-rich proteins.  
  Call Number Serial 52  
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Author Heyes, C. file  url
  Title Causes and consequences of imitation Type Journal Article
  Year (down) 2001 Publication Trends in Cognitive Sciences Abbreviated Journal Trends Cogn Sci  
  Volume 5 Issue 6 Pages 253-261  
  Keywords Imitation; Chimpanzees; Birds; Autism  
  Abstract Recent behavioural and neuroscientific research concerning imitation has revealed evidence of experience-dependent imitation in chimpanzees and birds, wide ranging imitation deficits in autism, and unintentional imitation in adult humans. This review examines these findings and also evaluates evidence of neonatal imitation and intentional imitation in infancy, and evidence suggesting that the left inferior frontal gyrus is specialized for imitation. At the theoretical level, the empirical findings support the view that the perceptual-motor translation that is a unique and defining property of imitation depends primarily on direct links between sensory and motor representations established through correlated experience of observing movements and carrying them out.  
  Call Number Serial 70  
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Author Chan, K.H.; Tam, J.S.; Peiris, J.S.; Seto, W.H.; Ng, M.H. file  url
  Title Epstein-Barr virus (EBV) infection in infancy Type Journal Article
  Year (down) 2001 Publication Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology Abbreviated Journal J Clin Virol  
  Volume 21 Issue 1 Pages 57-62  
  Keywords Antibodies, Viral/blood; Capsid/immunology; Epstein-Barr Virus Infections/blood/*epidemiology; Epstein-Barr Virus Nuclear Antigens/immunology; Female; Fetal Blood; Herpesvirus 4, Human/*immunology; Hong Kong/epidemiology; Humans; Infant, Newborn; Longitudinal Studies; Male; Seroepidemiologic Studies  
  Abstract BACKGROUND: Epstein-Barr virus (EBV) has been shown to be the cause of infectious mononucleosis (IM) and has more complicated associations with several malignant diseases. These EBV associated diseases provide a strong incentive for the development of an EBV vaccine. Most primary EBV infection during infancy and early childhood is mild or subclinical. Little is known about its infection in infancy. The pattern of EBV serological response during infancy may be important for vaccine management. OBJECTIVES: this study has served to clarify the epidemiology and serology of primary EBV infection during early infancy. STUDY DESIGN: longitudinal serum samples from 66 Hong Kong infants were tested for EBV antibodies by immunofluorescence. Cord blood and sequential serum samples from these infants were taken at birth and then at 4-month intervals up to 2 years of age. RESULTS: maternal antibodies were present at different levels in all cord blood specimens and in serum samples of 8 infants at 4-month of age. Evidenced by VCA-IgG seroconversion, 60.6% (40/66) infants were infected during the first 2 years of life. One episode occurred before 8 months of age but, thereafter and for the remaining 16 months of follow-up until the infants were 2 years of age, the infection occurred at essentially a constant rate affecting about 20% of the remaining seronegative infants every 4 months. CONCLUSIONS: the abrupt onset of the infection after a delay of 8 months is a remarkable feature of primary EBV infection during infancy, which implicates a protective role for maternal antibodies. Persisting maternal antibodies may additionally serve to contain the infection once it occurred. This may partly explain why, unlike during adolescence, primary EBV infection early in life is usually asymptomatic.  
  Call Number Serial 110  
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Author Pezeshki, S.R.; DeLaune, R.D.; Jugsujinda, A. file  url
  Title The effects of crude oil and the effectiveness of cleaner application following oiling on US Gulf of Mexico coastal marsh plants Type Journal Article
  Year (down) 2001 Publication Environmental Pollution (Barking, Essex : 1987) Abbreviated Journal Environ Pollut  
  Volume 112 Issue 3 Pages 483-489  
  Keywords Carbon Dioxide/analysis; Ecosystem; Fresh Water; Louisiana; Petroleum/*toxicity; Photosynthesis/drug effects/physiology; *Plant Development; Plant Leaves/drug effects/physiology; Plants/*drug effects; Random Allocation; Surface-Active Agents/*chemistry; Water Pollutants, Chemical/*toxicity  
  Abstract Field studies were conducted in two different marsh habitats in Louisiana coastal wetlands to evaluate the effects of oiling (using South Louisiana Crude oil, SLC) and the effectiveness of a shoreline cleaner (COREXIT 9580) in removing oil from plant canopies. The study sites represented two major marsh habitats; the brackish marsh site was covered by Spartina patens and the freshwater marsh was covered by Sagittaria lancifolia. Field studies were conducted in each habitat using replicated 5.8 m2 plots that were subjected to three treatments; oiled only, oiled + cleaner (cleaner was used 2 days after oiling), and a control. Plant gas exchange responses, survival, growth, and biomass accumulation were measured. Results indicated that oiling led to rapid reductions in leaf gas exchange rates in both species. However, both species in 'oiled + cleaned' plots displayed improved leaf conductance and CO2 fixation rates. Twelve weeks after treatment initiation, photosynthetic carbon fixation in both species had recovered to normal levels. Over the short-term, S. patens showed more sensitivity to oiling with SLC than S. lancifolia as was evident from the data of the number of live shoots and above-ground biomass. Above-ground biomass remained significantly lower than control in S. patens under 'oiled' and 'oiled + cleaned' treatments while it was comparable to controls in S. lancifolia. These studies indicated that the cleaner removed oil from marsh grasses and alleviated the short-term impact of oil on gas exchange function of the study plants. However, use of cleaner had no detectable effects on above-ground biomass production or regeneration at the end of the first growing season in S. patens. Similarly, no beneficial effects of cleaner on carbon fixation and number of live shoots were apparent beyond 12 weeks in S. lancifolia.  
  Call Number Serial 131  
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Author Gruber, O.; von Cramon, D.Y. file  url
  Title Domain-specific distribution of working memory processes along human prefrontal and parietal cortices: a functional magnetic resonance imaging study Type Journal Article
  Year (down) 2001 Publication Neuroscience Letters Abbreviated Journal Neurosci Lett  
  Volume 297 Issue 1 Pages 29-32  
  Keywords Adult; Female; Humans; Magnetic Resonance Imaging/methods; Male; Memory/*physiology; Parietal Lobe/*physiology; Prefrontal Cortex/*physiology; Vision, Ocular/physiology  
  Abstract This study reinvestigated the functional neuroanatomy of phonological and visual working memory in humans. Articulatory suppression was used to deprive the human subjects of species-specific verbal strategies in order to make the functional magnetic resonance imaging results more comparable to findings in non-human primates. Both phonological and visual working memory processes activated similar prefronto-parietal networks but were found to be differentially distributed along several cortical structures, in particular along the anterior and posterior parts of the intermediate frontal sulcus. These results suggest that a domain-specific topographical organization of neural working memory mechanisms in the primate brain is conserved in evolution. However, the findings also underline the critical dynamic influence that the additional availability of language may have on working memory processes and their functional implementation in the human brain.  
  Call Number Serial 142  
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Author Filaire, M.; Vacheron, J.J.; Vanneuville, G.; Poumarat, G.; Garcier, J.M.; Harouna, Y.; Guillot, M.; Terver, S.; Toumi, H.; Thierry, C. file  url
  Title Influence of the mode of load carriage on the static posture of the pelvic girdle and the thoracic and lumbar spine in vivo Type Journal Article
  Year (down) 2001 Publication Surgical and Radiologic Anatomy : SRA Abbreviated Journal Surg Radiol Anat  
  Volume 23 Issue 1 Pages 27-31  
  Keywords Adult; Humans; Intervertebral Disc Displacement/physiopathology; Lumbar Vertebrae/*physiology; Male; Pelvis/*physiology; Posture/*physiology; Reference Values; Thoracic Vertebrae/*physiology; Weight-Bearing/physiology  
  Abstract The influence of various modes of carrying a load of 16 kg (15.69 DaN) on the static positioning of the pelvic girdle and the thoracic and lumbar segments of the spine was examined in seven male subjects. The displacement of cutaneous markers attached to easily palpable skeletal landmarks was recorded using 4 CCD cameras; the data acquired were analysed using an optoelectronic technique (SAGA3). The subjects stood upright on an AMTI biomechanical force platform, from which the ground reaction forces enabled displacements of the centre of gravity axis and thus the moment of the mass carried to be determined. The modes of load carriage examined were: 1) in a case in the left hand; 2) in a case in the right hand; 3) equally in two cases; 4) on the head; 5) in a rucksack; and 6) in an anterior bag. The results showed displacements of the pelvic girdle, the caudal and cranial lumbar segments, and the caudal and cranial thoracic segments in the three orthogonal planes (sagittal, frontal and transverse). The influence of the moment created by the load was seen in the statokinesigrams. The use of external markers using an optoelectronic technique, in association with the ground reaction forces, enables the mode of load carriage to be determined. The results show that the influence of the moment exerted by the mode of load carriage on the gravity axis has important ergonomic consequences.  
  Call Number Serial 143  
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Author Yuan, W.; Parrish, C.R. file  url
doi  openurl
  Title Canine parvovirus capsid assembly and differences in mammalian and insect cells Type Journal Article
  Year (down) 2001 Publication Virology Abbreviated Journal Virology  
  Volume 279 Issue 2 Pages 546-557  
  Keywords Animals; Baculoviridae/genetics; Capsid/*physiology; Cell Line; Cell Nucleus/metabolism; Cytoplasm/metabolism; Dogs; Electrophoresis, Polyacrylamide Gel; Fluorescent Antibody Technique; Hydrogen-Ion Concentration; Mammals; Mutation; Parvovirus, Canine/genetics/*physiology; Recombinant Proteins/biosynthesis; Spodoptera; Time Factors; Transfection; Urea/pharmacology; Viral Proteins/biosynthesis/genetics; *Virus Assembly/drug effects  
  Abstract We examined the assembly processes of the capsid proteins of canine parvovirus (CPV) in mammalian and insect cells. In CPV-infected cells empty capsids assembled within 15 min, and then continued to form over the following 1 h, while full (DNA-containing) capsids were detected only after 60 min, and those accumulated slowly over several hours. In cells expressing VP1 and VP2 or only VP2, empty capsid formation was also efficient, but was slightly slower than that in infected cells. Small amounts of trimer forms of VP2 were detected in cells expressing wild type capsid proteins, but were not seen for mutants containing changes that prevented capsid assembly. CPV capsids accumulated in the cell nucleus, but mutant VP1 and VP2 proteins that did not assemble became distributed throughout the nucleus and the cytoplasm, irrespective of whether they were expressed as VP1 and VP2, or as VP2 only. Urea or pH treatment of empty capsids released dimer, trimer, or pentamer capsid protein combinations, while treatment of full capsids consistently released trimer and, in some cases, pentamer forms. When wild type or assembly-defective VP2 genes were expressed from recombinant baculoviruses in insect cells, most of the protein was recovered as noncapsid aggregates, and only a small proportion assembled into capsids. Both the assembled capsids and the noncapsid aggregates were seen primarily in the cytoplasm of the insect cells. The VP2 expressed in insect cells that was recovered in aggregates had an isoelectric point of about pH 6.3, while that recovered from assembled capsids had a pI of about 5.2, similar to that seen for the VP2 of capsids recovered from mammalian cells.  
  Call Number Serial 162  
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Author Amato, P.R. file  url
  Title Children of divorce in the 1990s: an update of the Amato and Keith (1991) meta-analysis Type Journal Article
  Year (down) 2001 Publication Journal of Family Psychology : JFP : Journal of the Division of Family Psychology of the American Psychological Association (Division 43) Abbreviated Journal J Fam Psychol  
  Volume 15 Issue 3 Pages 355-370  
  Keywords Adolescent; Adult; Age Factors; Child; Child Psychology; Child, Preschool; Divorce--psychology; Educational Status; Female; Humans; Male; Marriage--psychology; Mental Health; Research Design; Self Concept; Sex Factors; Social Adjustment; United States--epidemiology  
  Abstract The present study updates the P. R. Amato and B. Keith (1991) meta-analysis of children and divorce with a new analysis of 67 studies published in the 1990s. Compared with children with continuously married parents, children with divorced parents continued to score significantly lower on measures of academic achievement, conduct, psychological adjustment, self-concept, and social relations. After controlling for study characteristics, curvilinear trends with respect to decade of publication were present for academic achievement, psychological well-being, self-concept, and social relations. For these outcomes, the gap between children with divorced and married parents decreased during the 1980s and increased again during the 1990s.  
  Call Number Serial 276  
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Author Hallab, N.J.; Bundy, K.J.; O'Connor, K.; Moses, R.L.; Jacobs, J.J. file  url
doi  openurl
  Title Evaluation of metallic and polymeric biomaterial surface energy and surface roughness characteristics for directed cell adhesion Type Journal Article
  Year (down) 2001 Publication Tissue Engineering Abbreviated Journal Tissue Eng  
  Volume 7 Issue 1 Pages 55-71  
  Keywords 3T3 Cells; Animals; *Biocompatible Materials; Biomedical Engineering/*methods; *Cell Adhesion; Electrophoresis, Polyacrylamide Gel; Extracellular Matrix Proteins/chemistry/secretion; Fibroblasts/metabolism/secretion/ultrastructure; Humans; Metals/*chemistry; Mice; Polymers/*chemistry; Statistics as Topic; Surface Properties  
  Abstract Directed cell adhesion remains an important goal of implant and tissue engineering technology. In this study, surface energy and surface roughness were investigated to ascertain which of these properties show more overall influence on biomaterial-cell adhesion and colonization. Jet impingement was used to quantify cellular adhesion strength. Cellular proliferation and extracellular matrix secretion were used to characterize colonization of 3T3MC fibroblasts on: HS25 (a cobalt based implant alloy, ASTM F75), 316L stainless steel, Ti-6Al4V (a titanium implant alloy), commercially pure tantalum (Ta), polytetrafluoroethylene (PTFE), silicone rubber (SR), and high-density polyethylene (HDPE). The metals exhibited a nearly five-fold greater adhesion strength than the polymeric materials tested. Generally, surface energy was proportional to cellular adhesion strength. Only polymeric materials demonstrated significant increased adhesion strength associated with increased surface roughness. Cellular adhesion on metals demonstrated a linear correlation with surface energy. Less than half as much cellular proliferation was detected on polymeric materials compared to the metals. However the polymers tested demonstrated greater than twice the amount of secreted extracellular matrix (ECM) proteins on a per cell basis than the metallic materials. Thus, surface energy may be a more important determinant of cell adhesion and proliferation, and may be more useful than surface roughness for directing cell adhesion and cell colonization onto engineered tissue scaffoldings.  
  Call Number Serial 428  
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Author Denner, E.B.; Mark, B.; Busse, H.J.; Turkiewicz, M.; Lubitz, W. file  url
doi  openurl
  Title Psychrobacter proteolyticus sp. nov., a psychrotrophic, halotolerant bacterium isolated from the Antarctic krill Euphausia superba Dana, excreting a cold-adapted metalloprotease Type Journal Article
  Year (down) 2001 Publication Systematic and Applied Microbiology Abbreviated Journal Syst Appl Microbiol  
  Volume 24 Issue 1 Pages 44-53  
  Keywords Adaptation, Biological/*physiology; Animals; Antarctic Regions; Bacterial Proteins/isolation & purification; Bacterial Typing Techniques; Cold Temperature; Crustacea/*microbiology; DNA, Ribosomal/genetics; Drug Resistance, Microbial; Fatty Acids/analysis; Gammaproteobacteria/classification/enzymology/*isolation & purification; Gram-Negative Aerobic Rods and Cocci/enzymology/*isolation & purification; Metalloendopeptidases/*secretion; Molecular Sequence Data; RNA, Ribosomal, 16S/genetics; Salts/pharmacology  
  Abstract An Antarctic marine bacterium (strain 116) excreting an extracellular cold-adapted metalloprotease was subjected to a detailed polyphasic taxonomic investigation. Strain 116 was previously isolated from the stomach of a specimen of the Antarctic krill Euphasia superba Dana and tentatively characterized as Sphingomonas paucimobilis 116. The 16S rDNA sequence analysis showed that the strain is in fact related to species of the genus Psychrobacter, next to Psychrobacter glacincola (97.4% similarity). Sequence similarities between strain 116 and other Psychrobacter species ranged from 96.9% (with P. urativorans) to 95.4% (with P. immobilis). Key phenotypic characteristics as well as chemotaxonomic features of the bacterium were congruent with the description of the genus Psychrobacter i.e. cells were strictly aerobic, strongly oxidase-positive, psychrotrophic, halotolerant, gram-negative non-motile coccobacilli, with ubiquinone-8 as the main respiratory lipoquinone and 18:1 cis 9, 16:1 cis and 17:1 (omega8c being the predominant cellular fatty acids. The G+C content of the DNA was 43.6 mol%. DNA-DNA hybridization studies showed that the relatedness between strain 116 and Psychrobacter glacinola is only 62.2%. Further differences were apparent in whole-cell SDS-PAGE protein pattern, cellular fatty acid profile and in a number of physiological and biochemical characteristics as well as in enzymatic activities. Tolerance to 5% bile salts, nitrate reduction, citrate utilization, acid production from carbohydrates, alkaline phosphatase, acid phosphatase, C4 esterase, C14 lipase and valine arylamidase were found to differentiate strain 116 from Psychrobacter glacincola. On the basis of this phenotypic and molecular evidences, strain 116, previously known as Sphingomonas paucimobilis 116, was recognized as a new species of the genus Psychrobacter for which the name Psychrobacter proteolyticus is proposed. Strain 116 has been deposited in the Collection de l'Institut Pasteur, France, as CIP106830T and in the Deutsche Sammlung von Mikroorganismen and Zellkulturen, as DSM13887.  
  Call Number Serial 436  
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