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Author: Zhan, Q.; Fan, S.; Bae, I.; Guillouf, C.; Liebermann, D.A.; O'Connor, P.M.; Fornace, A.J.J.

Description: DNA-damaging agents such as ionizing radiation (IR) activate the tumor suppressor p53 and in some cases can cause apoptosis. M1 cells, which do not express the endogenous tumor suppressor gene p53, undergo apoptosis following activation of a temperature sensitive p53 transgene, where it has been shown that bax, an important mediator of apoptosis, is a p53 target gene (Selvakumaran et al, Oncogene 9, 1791-8, 1994). Since p53 can function as a transcription factor after activation by IR, the genetic response to this stress was examined in a panel of human cells with defined p53 status. Like the p53-regulated gene gadd45, bax was rapidly induced, as measured by increased mRNA levels, in the p53 wt (wild type) human myeloid line ML-1, and it was not induced in cells lacking functional p53. However, unlike other p53-regulated genes, bax was only induced in p53 wt cells in which IR also triggered apoptosis. In the case of bcl2, which opposes bax function, mRNA levels were reduced in ML-1 cells after IR. Thus, bax appears to be an unique p53-regulated gene in that its induction by IR not only requires functional p53 but also requires that the cells be apoptosis "proficient."

Title: Induction of bax by genotoxic stress in human cells correlates with normal p53 status and apoptosis

Subject headings: Apoptosis/*genetics; Gene Expression Regulation/*drug effects/genetics/radiation effects; *Genes, p53; Humans; Mutagens/*toxicity; Neoplasms/genetics; Proto-Oncogene Proteins/*genetics; Proto-Oncogene Proteins c-bcl-2; Tumor Cells, Cultured; bcl-2-Associated X Protein

Year: 1994

Publication: Oncogene

Volume: 9

Issue: 12

Pages: 3743-3751

Full text: http://europepmc.org/abstract/med/7970735

Cited by: https://scholar.google.com/scholar?cites=6269641274711173672&as_sdt=1000005&sciodt=0,16&hl=en

Format: Journal Article

ISSN: 0950-9232

ISBN:
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Author: Zhang, B.; Stewart, B.

Description: The Drosophila larval neuromuscular junction (NMJ) shares many structural and functional similarities to synapses in other animals, including humans. These include the basic feature of synaptic transmission, as well as the molecular mechanisms regulating the synaptic vesicle cycle. Because of its large size, easy accessibility, and the well-characterized genetics, the fly NMJ remains an excellent model system for dissecting the cellular and molecular mechanisms of synaptic transmission. This protocol describes the steps for performing intracellular recording from fly larval body-wall muscles and explains how to record and analyze spontaneous and evoked synaptic potentials. Methods used include larval dissection ("filleting"), identification of muscle fibers and their innervating nerves, the use of a micromanipulator and microelectrode in penetrating the muscle membrane, and nerve stimulation to evoke synaptic potentials.

Title: Electrophysiological recording from Drosophila larval body-wall muscles

Subject headings: Animals; Cytosol/physiology; Drosophila/*physiology; Electrophysiology/instrumentation/*methods; Muscles/physiology; Neuromuscular Junction/physiology

Year: 2010

Publication: Cold Spring Harbor Protocols

Volume: 2010

Issue: 9

Pages: pdb.prot5487

Full text: http://cshprotocols.cshlp.org/content/2010/9/pdb.prot5487.short

Cited by: https://scholar.google.com/scholar?cites=8497526474665901244&as_sdt=1000005&sciodt=0,16&hl=en

Format: Journal Article

ISSN: 1559-6095

ISBN:
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Author: Zhang, G.; Ma, X.; Niu, F.; Dong, M.; Feng, H.; An, L.; Cheng, G.

Description: The Qinghai-Tibet Plateau represents a unique permafrost environment, being a result of high elevation caused by land uplift. And the urgency was that plateau permafrost was degrading rapidly under the current predicted climatic warming scenarios. Hence, the permafrost there was sampled to recover alkaliphilic bacteria populations. The viable bacteria on modified PYGV agar were varied between 10(2) and 10(5 )CFU/g of dry soil. Forty-eight strains were gained from 18 samples. Through amplified ribosomal DNA restriction analysis (ARDRA) and phylogenetic analyses, these isolates fell into three categories: high G + C gram positive bacteria (82.3%), low G + C gram positive bacteria (7.2%), and gram negative alpha-proteobacteria (10.5%). The strains could grow at pH values ranging from 6.5 to 10.5 with optimum pH in the range of 9-9.5. Their growth temperatures were below 37 degrees C and the optima ranging from 10 to 15 degrees C. All strains grew well when NaCl concentration was below 15%. These results indicate that there are populations of nonhalophilic alkaliphilic psychrotolerant bacteria within the permafrost of the Qinhai-Tibet plateau. The abilities of many of the strains to produce extracellular protease, amylase and cellulase suggest that they might be of potential value for biotechnological exploitation.

Title: Diversity and distribution of alkaliphilic psychrotolerant bacteria in the Qinghai-Tibet Plateau permafrost region

Subject headings: *Adaptation, Physiological; Bacteria/classification/*isolation & purification; Base Sequence; Culture Media; DNA Primers; Phylogeny; Species Specificity

Year: 2007

Publication: Extremophiles : Life Under Extreme Conditions

Volume: 11

Issue: 3

Pages: 415-424

Full text: http://link.springer.com/article/10.1007/s00792-006-0055-9

Cited by: https://scholar.google.com/scholar?cites=7119702762095934761&as_sdt=1000005&sciodt=0,16&hl=en

Format: Journal Article

ISSN: 1431-0651

ISBN:
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Author: Zhang, G.; Niu, F.; Ma, X.; Liu, W.; Dong, M.; Feng, H.; An, L.; Cheng, G.

Description: The Qinghai-Tibet Plateau in east Asia is a unique and important permafrost environment. However, its microbiology remains largely unexplored to date. In this study, sediment samples were collected from the Qinghai-Tibet Plateau permafrost region, bacteria isolation procedures were performed 8 times, and the samples incubated at 4 degrees C for nearly 3 months. The number of colony forming units (cfu) ranged from 0 to 10(7)/(g dry soil). The quantity of culturable bacteria grew exponentially within the first few weeks, and then slowed gradually to a plateau. Phylogenetic analyses indicated that all the isolates fell into 6 categories: high G+C Gram-positive bacteria, low G+C Gram-positive bacteria, alpha-Proteobacteria, beta-Proteobacteria, gamma-Proteobacteria, and Cytophaga-Flavobacterium-Bacteroides group bacteria. The isolates belong to 19 genera, but the genera Arthrobacter and Pseudomonas were predominant. With the increase in incubation time, the isolated populations changed in terms of both species and their respective quantities. Of the 33 analyzed isolates, 9 isolates related to 8 genera might be new taxa. These results suggest that the Qinghai-Tibet Plateau permafrost region is a specific ecologic niche that accommodates an original microbial assemblage.

Title: Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region

Subject headings: Altitude; Bacteria/classification/*genetics/isolation & purification; Biodiversity; *Genetic Variation; Molecular Sequence Data; *Phylogeny; RNA, Ribosomal, 16S/genetics; Sequence Analysis, DNA; Tibet

Year: 2007

Publication: Canadian Journal of Microbiology

Volume: 53

Issue: 8

Pages: 1000-1010

Full text: http://www.nrcresearchpress.com/doi/abs/10.1139/W07-031#.VaU1m2A-DJg

Cited by: https://scholar.google.com/scholar?cites=10625008363275178677&as_sdt=1000005&sciodt=0,16&hl=en

Format: Journal Article

ISSN: 0008-4166

ISBN:
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Author: Zhang, H.

Description: The combustion of light hydrocarbons finds an important application in volatile organic compounds (VOCs) abatement. Catalytic combustion is interesting in this domain, because it may be carried out at relatively low temperatures and under large air excess. For this purpose, precious metal-based catalysts are very performing, but the quest for a lower-cost alternative solution is necessary. In this field, conducting mixed oxides such as perovskites are good candidates, especially when they are electrochemically promoted. The present work shows that electrochemical promotion of catalytic activity (NEMCA effect) in propene total combustion can be carried out with La0.8Sr0.2Co0.8Fe0.2O3, an electron-conducting perovskite-type oxide, deposited on YSZ.

Title: Oxygen sorption and catalytic properties of La1$minus;xSrxCo1$minus;yFeyO3 Perovskite-type oxides

Subject headings: catalytic combustion electrochemical promotion EPOC NEMCA propene VOC perovskite

Year: 1990

Publication: Journal of Catalysis

Volume: 121

Issue: 2

Pages: 432-440

Full text: http://link.springer.com/article/10.1023/B:CATL.0000030117.00142.3d

Cited by: https://scholar.google.com/scholar?cites=9581446771238481081&as_sdt=1000005&sciodt=0,16&hl=en

Format: Journal Article

ISSN: 0021-9517

ISBN:
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Author: Zhang, H.; Xu, M.; Zhang, F.

Description: Rice (Oryza sativa L.), wheat (Triticum aestivum L.) and maize (Zea mays L.) are the main crops grown in China. Applying organic manures is an important practice in sustaining soil fertility and agricultural productivity in these cropping systems. The current paper presents the effects of manure application on grain yields in nine long-term experiments that consist of one continuous maize, four wheat–maize and four rice-based cropping systems across a wide range of agro-ecological regions in China. The study shows that regular manure application can increase soil organic carbon (SOC) and grain yield across all the sites. Overall, regular use of manure results in larger increases in SOC in the maize and wheat–maize systems than in the rice-based systems. Application of manure tends to increase the grain yield in the maize and wheat–maize systems during the final years, but increases the grain yield in the rice-based systems during the initial years of the long-term experiments. There is only one site that shows significant improvement in the yield trend in association with the application of manure. The effects of manure on yield trends are probably determined by the initial yield and/or the ‘organic C effect’ that may cause gradual improvements in SOC and soil physical properties.

Title: Long-term effects of manure application on grain yield under different cropping systems and ecological conditions in China

Subject headings: Rice; Cropping systems; Manures

Year: 2009

Publication: The Journal of Agricultural Science

Volume: 147

Issue: 01

Pages: 31

Full text: http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=3103988&fileId=S0021859608008265

Cited by: https://scholar.google.com/scholar?cites=298389179374380047&as_sdt=1000005&sciodt=0,16&hl=en

Format: Journal Article

ISSN: 0021-8596

ISBN:
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Author: Zhang, L.-M.; Wang, M.; Prosser, J.I.; Zheng, Y.-M.; He, J.-Z.

Description: To determine the abundance and distribution of bacterial and archaeal ammonia oxidizers in alpine and permafrost soils, 12 soils at altitudes of 4000-6550 m above sea level (m a.s.l.) were collected from the northern slope of the Mount Everest (Tibetan Plateau), where the permanent snow line is at 5800-6000 m a.s.l. Communities were characterized by real-time PCR and clone sequencing by targeting on amoA genes, which putatively encode ammonia monooxygenase subunit A. Archaeal amoA abundance was greater than bacterial amoA abundance in lower altitude soils (<or=5400 m a.s.l.), but this situation was reversed in higher altitude soils (>or=5700 m a.s.l.). Both archaeal and bacterial amoA abundance decreased abruptly in higher altitude soils. Communities shifted from a Nitrosospira amoA cluster 3a-dominated ammonia-oxidizing bacteria community in lower altitude soils to communities dominated by a newly designated Nitrosospira ME and cluster 2-related groups and Nitrosomonas cluster 6 in higher altitude soils. All archaeal amoA sequences fell within soil and sediment clusters, and the proportions of the major archaeal amoA clusters changed between the lower altitude and the higher altitude soils. These findings imply that the shift in the relative abundance and community structure of archaeal and bacterial ammonia oxidizers may result from selection of organisms adapted to altitude-dependent environmental factors in elevated soils.

Title: Altitude ammonia-oxidizing bacteria and archaea in soils of Mount Everest

Subject headings: Altitude; Ammonia/*metabolism; Archaea/classification/*genetics; Bacteria/classification/*genetics; Biodiversity; Cloning, Molecular; DNA, Archaeal/genetics; DNA, Bacterial/genetics; Ecosystem; Genes, Archaeal; Genes, Bacterial; Oxidoreductases/genetics; Sequence Analysis, DNA; Soil/analysis; *Soil Microbiology

Year: 2009

Publication: FEMS Microbiology Ecology

Volume: 70

Issue: 2

Pages: 52-61

Full text: http://femsec.oxfordjournals.org/content/70/2/208.abstract

Cited by: https://scholar.google.com/scholar?cites=14971675169724060777&as_sdt=1000005&sciodt=0,16&hl=en

Format: Journal Article

ISSN: 0168-6496

ISBN:
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Author: Zhang, L.; Lyons, L.; Newhouse, J.; Zhang, Z.; Straughan, M.; Chen, Z.; Amine, K.; Hamers, R.J.; West, R.

Description: Alkylsilane ethers, containing one or three carbon spacer groups between the silicon atom and oligo(ethylene oxide) moiety, were designed and synthesized. These compounds are non-hydrolyzable and less flammable than their alkoxysilane counterparts. A full cell test using them as electrolyte solvents showed good cycling performance in lithium-ion batteries.

Title: Synthesis and characterization of alkylsilane ethers with oligo(ethylene oxide) substituents for safe electrolytes in lithium-ion batteries

Subject headings: Alkylsilane ethers; Full cell test

Year: 2010

Publication: Journal of Materials Chemistry

Volume: 20

Issue: 38

Pages: 8224

Full text: http://pubs.rsc.org/en/content/articlelanding/2010/jm/c0jm01596b#!divAbstract

Cited by: https://scholar.google.com/scholar?cites=9542307099382318582&as_sdt=1000005&sciodt=0,16&hl=en

Format: Journal Article

ISSN: 0959-9428

ISBN:
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Author: Zhang, Q.; Riechers, D.E.

Description: Proteomic methods such as two-dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry, as well as immunoblotting, were used to identify herbicide safener-induced proteins in the coleoptile of Triticum tauschii, a diploid wheat containing the D genome also found in the cultivated, hexaploid wheat Triticum aestivum. The herbicide safener fluxofenim dramatically increased protein abundance in the molecular weight (M(r)) range of 24 to 30 kDa, as well as a few higher M(r) proteins, in the coleoptile of T. tauschii seedlings. In total, twenty proteins were identified in this study. Eleven proteins were highly safener induced and only weakly expressed in the control; seven proteins were new safener induced proteins that were not detected in the control. Two other proteins were constitutively expressed in both the control and safener-treated coleoptiles. Among the eighteen inducible proteins, fifteen were glutathione S-transferase (GST) subunits that fall into three subclasses: eight proteins were from the tau subclass, six proteins were from the phi subclass, and one protein was from the lambda class. Another three safener inducible proteins showed homology to the aldo/keto reductase family and with proteins that have roles in glycolysis and the Krebs cycle. Two constitutively expressed proteins were identified, one having highest homology to the dehydroascorbate reductase subclass of GSTs and one with an ascorbate peroxidase. Immunoblot analyses, using two different antisera raised against the same GST protein but differing in their specificity, were used to further characterize the GST proteins expressed in response to safener treatment. Results from immunoblotting, combined with mass spectral analysis, showed that post-translational modification of GST proteins in control and safener-treated coleoptiles may occur.

Title: Proteomic characterization of herbicide safener-induced proteins in the coleoptile of Triticum tauschii seedlings

Subject headings: Chromatography, Liquid; Cotyledon/chemistry/metabolism; Databases as Topic; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Glutathione Transferase/metabolism; Herbicides/chemistry/*metabolism; Hydrogen-Ion Concentration; Image Processing, Computer-Assisted; Immunoblotting; Mass Spectrometry; Poaceae/metabolism; Proteins/chemistry; Proteome; Proteomics/*methods; Seedling/metabolism; Seeds/*metabolism; Silver Staining; Tritium/*metabolism

Year: 2004

Publication: Proteomics

Volume: 4

Issue: 7

Pages: 2058-2071

Full text: http://onlinelibrary.wiley.com/doi/10.1002/pmic.200300733/abstract

Cited by: https://scholar.google.com/scholar?cites=15155665350266941173&as_sdt=1000005&sciodt=0,16&hl=en

Format: Journal Article

ISSN: 1615-9853

ISBN:
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Author: Zhang, Q.; Yu, Y.-P.; Ye, Y.-L.; Zhang, J.-T.; Zhang, W.-P.; Wei, E.-Q.

Description: In the present study, we investigated the spatiotemporal properties of locomotor activity after administration of CNS sedatives (pentobarbital and diazepam) and stimulants (theophylline and caffeine) in an open field test. The absolute and relative distances traveled in central or peripheral regions within 2 h were analyzed. We found that both pentobarbital and diazepam increased total travel distances, especially within the initial 30 min, when traveling was mainly in the peripheral region. Pentobarbital induced this hyperactivity at higher doses (maximum at 30 mg/kg); while diazepam at higher doses (4 and 8 mg/kg) mainly decreased the traveled distance during 0-1 h but increased that in the periphery during 1-2 h. On the other hand, both theophylline and caffeine generally increased the traveled distance in the central region; this effect lasted longer with increasing dose. Caffeine increased the traveled distance at lower doses (maximum at 10 mg/kg) but decreased it at higher doses (30 and 100 mg/kg) during the initial 1 h. Theophylline exhibited a similar but smaller decrease at higher doses. Thus, we revealed the spatiotemporal properties that sedatives decrease central locomotion but induce a dose-related peripheral hyperactivity while stimulants induce central hyperactivity with a bell-shaped dose-response relation.

Title: Spatiotemporal properties of locomotor activity after administration of central nervous stimulants and sedatives in mice

Subject headings: Animals; Central Nervous System Stimulants/*pharmacology; Hypnotics and Sedatives/*pharmacology; Locomotion/*drug effects; Male; Mice

Year: 2011

Publication: Pharmacology, Biochemistry, and Behavior

Volume: 97

Issue: 3

Pages: 577-585

Full text: http://www.sciencedirect.com/science/article/pii/S0091305710002753

Cited by: https://scholar.google.com/scholar?cites=10939579293522633942&as_sdt=1000005&sciodt=0,16&hl=en

Format: Journal Article

ISSN: 0091-3057

ISBN:





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