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Author (up) Dzidic, M.; Abrahamsson, T.R.; Artacho, A.; Bjorksten, B.; Collado, M.C.; Mira, A.; Jenmalm, M.C. file  url
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  Title Aberrant IgA responses to the gut microbiota during infancy precede asthma and allergy development Type Journal Article
  Year 2017 Publication The Journal of Allergy and Clinical Immunology Abbreviated Journal J Allergy Clin Immunol  
  Volume 139 Issue 3 Pages 1017-1025.e14  
  Keywords Bacteria/isolation & purification; Bacterial Load; Child; Child, Preschool; Feces/*microbiology; Female; *Gastrointestinal Microbiome; Humans; Hypersensitivity/*immunology/*microbiology; Immunoglobulin A/*immunology; Infant; Male; Allergic disease; IgA index; IgA recognition patterns; asthma; childhood; gut microbiota; microbiome composition; secretory IgA  
  Abstract BACKGROUND: Although a reduced gut microbiota diversity and low mucosal total IgA levels in infancy have been associated with allergy development, IgA responses to the gut microbiota have not yet been studied. OBJECTIVE: We sought to determine the proportions of IgA coating together with the characterization of the dominant bacteria, bound to IgA or not, in infant stool samples in relation to allergy development. METHODS: A combination of flow cytometric cell sorting and deep sequencing of the 16S rDNA gene was used to characterize the bacterial recognition patterns by IgA in stool samples collected at 1 and 12 months of age from children staying healthy or having allergic symptoms up to 7 years of age. RESULTS: The children with allergic manifestations, particularly asthma, during childhood had a lower proportion of IgA bound to fecal bacteria at 12 months of age compared with healthy children. These alterations cannot be attributed to differences in IgA levels or bacterial load between the 2 groups. Moreover, the bacterial targets of early IgA responses (including coating of the Bacteroides genus), as well as IgA recognition patterns, differed between healthy children and children with allergic manifestations. Altered IgA recognition patterns in children with allergy were observed already at 1 month of age, when the IgA antibodies are predominantly maternally derived in breast-fed children. CONCLUSION: An aberrant IgA responsiveness to the gut microbiota during infancy precedes asthma and allergy development, possibly indicating an impaired mucosal barrier function in allergic children.  
  Call Number Serial 1933  
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Author (up) Kampfer, P.; Albrecht, A.; Buczolits, S.; Busse, H.-J. file  url
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  Title Psychrobacter faecalis sp. nov., a new species from a bioaerosol originating from pigeon faeces Type Journal Article
  Year 2002 Publication Systematic and Applied Microbiology Abbreviated Journal Syst Appl Microbiol  
  Volume 25 Issue 1 Pages 31-36  
  Keywords Aerosols; *Air Microbiology; Air Pollution; Animals; Columbidae/*microbiology; Fatty Acids/analysis; Feces/*microbiology; Gammaproteobacteria/genetics/*isolation & purification; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Ribosomal, 16S/analysis/classification; Sequence Alignment  
  Abstract The taxonomy of strain Iso-46T isolated from a bioaerosol generated by cleaning of a pigeon faeces contaminated room was investigated in a polyphasic approach. The beige pigmented Gram-negative, oxidase-negative organism contained a quinone system with mainly ubiquinone Q-8, and the polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, beside some hitherto uncharacterized phospholipids. Major polyamines were spermidine and putrescine and also small amounts of cadaverine. The analysis of the fatty acids revealed 3-OH 12:0 and 3-OH 14:0 (within summed feature 3) as hydroxylated fatty acids. These chemotaxonomic characteristics suggest that the strain belongs to the gamma-subclass of the Proteobacteria namely into the genus Psychrobacter. Analysis of the 16S rRNA gene supported the allocation into the genus Psychrobacter, but showing similarities to all described species of this genus lower than 97%. Iso-46T was able to grow on MacConkey agar and other high nutrient containing media within a temperature range of 4 degrees C to 36 degrees C. On the basis of nutritional and further physiological features, a clear differentiation from all other Psychrobacter species was possible. For these reasons it is proposed to create a new species with the name Psychrobacter faecalis sp. nov.  
  Call Number Serial 435  
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