more information
Search within Results:

Select All    Deselect All
 |   | 
Details
   print
  Records Links
Author (up) Downing, K.J.; Thomson, J.A. file  url
openurl 
  Title Introduction of the Serratia marcescens chiA gene into an endophytic Pseudomonas fluorescens for the biocontrol of phytopathogenic fungi Type Journal Article
  Year 2000 Publication Canadian Journal of Microbiology Abbreviated Journal Can J Microbiol  
  Volume 46 Issue 4 Pages 363-369  
  Keywords Chitinases/*genetics/metabolism; DNA-Binding Proteins/genetics/metabolism; Escherichia coli/genetics; Fabaceae/microbiology; *Pest Control, Biological; Plant Diseases/microbiology; Plants, Medicinal; Plasmids/genetics; Polymerase Chain Reaction/methods; Promoter Regions, Genetic; Pseudomonas fluorescens/*enzymology/*genetics/growth & development/isolation & purification; Repressor Proteins/genetics/metabolism; Rhizoctonia/*growth & development; *Saccharomyces cerevisiae Proteins; Serratia marcescens/enzymology/*genetics; *Telomere-Binding Proteins  
  Abstract An endophytic strain of Pseudomonas fluorescens was isolated from micropropagated apple plantlets and introduced into beans (Phaseolus vulgaris) via their root tips. It was shown to be present as an endophyte in the roots at a level of 1.2 x 10(5) CFU/g fresh weight. The gene coding for the major chitinase of Serratia marcescens, chiA, was cloned under the control of the tac promoter into the broad-host-range plasmid pKT240 and the integration vector pJFF350. Pseudomonas fluorescens carrying tacchiA either on the plasmid or integrated into the chromosome is an effective biocontrol agent of the phytopathogenic fungus Rhizoctonia solani on bean seedlings under plant growth chamber conditions.  
  Call Number Serial 1662  
Permanent link to this record
 

 
Author (up) Maslunka, C.; Gurtler, V.; Carr, E.L.; Seviour, R.J. file  url
doi  openurl
  Title Unique organization of the 16S-23S intergenic spacer regions of strains of Acinetobacter baylyi provides a means for its identification from other Acinetobacter species Type Journal Article
  Year 2008 Publication Journal of Microbiological Methods Abbreviated Journal J Microbiol Methods  
  Volume 73 Issue 3 Pages 227-236  
  Keywords Acinetobacter/*classification/*genetics; DNA Primers/genetics; DNA, Bacterial/chemistry/*genetics; DNA, Ribosomal Spacer/chemistry/*genetics; Molecular Sequence Data; Polymerase Chain Reaction/methods; Sequence Analysis, DNA  
  Abstract This paper extends an earlier report on rrn operon characteristics in members of the genus Acinetobacter. It describes a systematic approach towards developing and validating a protocol for elucidating how the intergenic spacer regions (ISR) in Acinetobacter baylyi strains are organized and allows the numbers of long and short ISRs to be determined. Experimental data confirmed the in silico predictions based on available A. baylyi rrn sequence data. All were shown to possess three long ISRs and 4 short ISRs, differing in most cases in length by about 90nt. However, the ISR arrangement in A. baylyi strain 93A2 was different. Although it also possessed 4 SISRs and three LISRs, their length difference was less (39nt) which was confirmed from its ISR sequence data. Primer sets for PCR identification of A. baylyi could then be determined. Applying the same approach to other species of Acinetobacter showed none shared the same ISR organization as A. baylyi. Its value in typing members of this genus is discussed.  
  Call Number Serial 116  
Permanent link to this record
Select All    Deselect All
 |   | 
Details
   print

Save Citations: